BIOCHEM C785 2 OA readiness check(Latest update)

BIOCHEM C785 2 OA readiness check(Latest update 2021)

What is the complementary sequence to 3’ ATG CGA ATA 5’? (0/1 Points)           

                3’ TAT TCG CAT 5’

3’ TAC GCT TAT 5’            

The        correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching

or            Pairing” You have to remember to pay attention to your numbers as well as your

lett         ers (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’

TA           C GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so

mir         ror answer to give correct answer: 3’ TAT TCG CAT 5’ If you chose 3' TAC GCT TAT 5',

this         is incorrect. Although the nucleotides are in the correct order, the 5' and 3' are in

the         wrong place. DNA strands that are complementary run in opposite directions. This

me         ans that if one strand starts with a 3', the the complementary strand must start with

a 5'         . If none of the answer choices give the correct answer in 5' ---> 3', then you need to

writ        e its mirror, which will run from 3' ---> 5'.

                3’ GAT AGC ATA 5’

5’ ATA AGC GTA 3’

2

Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’?

(1/1 Points)

  Tyr Val Tyr

Ile Gln Ile

Ile Asp Val

The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are noncomplementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNAMirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' ---> 3')

The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile

Ser His Gln

4

Which of the following are the correct components for a PCR reaction?

 (1/1 Points) dNTPs, Primer, RNA Polymerase, template RNA

dNTPs, Primer, DNA Polymerase, template DNA

The correct answer is dNTPs, Primer, DNA Polymerase, template DNA. Notice that all components are about DNA. The Primer is even a DNA Primer.  ATP, Primer, mRNA polymerase; template mRNA

The correct answer is silent mutation. The nucleotide sequence changes, but it codes for the same amino acid. The coding sequence 5' TAC 3' corresponds to the mRNA sequence 5' UAC 3' (Tyr), and the coding sequence 5' TAT 3' corresponds to the mRNA sequence 5' UAU 3' (Tyr). Since the C changed to at T, this is a point mutation. If the point mutation results in the same amino acid in the new sequence as in the original sequence, the point mutation is a silent mutation.

Missense

Nonsense

Insertion

8

 Huntington's is an autosomal dominant disorder. Which of the following chromosome pairs would be inherited by a person with Huntington's Disease? (Yellow box = Dominant allele, Green box = Recessive allele)

(1/1 Points)

Option 1

The correct answer is Option 1 because an autosomal dominant disorder would be inherited on numbered chromosomes, not sex chromosomes X or Y. Also, at least one dominant allele (yellow box) needs to be present for the individual to have the dominant disease.