Question 2 (30 pts) You have two cultures of bacteria that you want to use for incubation experiments examining rates of a microbially mediated reaction
Question #2 (30 pts) You have two cultures of bacteria that you want to use for incubation experiments examining rates of a microbially mediated reaction. You need to “grow them up†before initiating the experiment to make sure that bacterial biomass is sufficient to measure a discernable change in the concentration of a reaction product during the experiments so that you can calculate a rate. Culture A has a doubling time of 15 minutes and culture B has a doubling time of 45 minutes. In the initial cultures (e.g., time zero) culture A has a cell abundance of 100 cells/ml while culture B has a cell abundance of 200 cells/ml. Calculate cell abundance over time and prepare graphs showing the growth of each culture. For your experiments, you require at least 109 cells/mL in order to measure the rate process of interest. When will each culture be ready to conduct the experiments? Which culture will be ready first? Hint: Put the equations into Microsoft Excel (or whatever spread sheet program you use) to generate and plot the data. I will be grading the quality/proper construction of your plots, so make sure you have the right variables on the x and y axes and be sure they are labeled properly.